γδ T cells control gut pathology in a chronic inflammatory model of colorectal cancer

Abstract Gut gd T cells are important intraepithelial lymphocytes with critical roles in maintenance of gut barrier integrity against infection and cancer, through unknown mechanisms. In addition to their role function, absence has been reported lead faster polyp formation an inflammation unassociated model colorectal cancer (CRC). The significant linkage between Inflammatory Bowel Disease CRC is strong relevance translational research, it necessitates a deeper understanding the underlying cell-molecular mechanism causing associated CRC. new disease-relevant, chronic Colorectal (CRC) model, we observed enhanced colon pathology for both mice lacking cells. RNAseq analysis from TCR d−/− treated AOM-DSS revealed early profound dysregulated transcriptional landscape reduced tumor suppressive genes, upregulated induction epithelial mesenchymal transition increased expression inflammatory markers. As tumorigenesis progressed, colons harbored potentially immunosuppressive CD4 layer but not lamina propria. Consistently, cell dependent tissue signature identified positively correlated different stages adenocarcinoma patients. Taken together, demonstrate that display broad functions homeostasis by controlling immune functions, maintain